Shubham Thakur

Energetic Determinantsof Expanded PAM Recognitionin Engineered FnCas9

Abstract Expanding protospacer-adjacent motif (PAM) compatibility while preserving specificity remains a central challenge in CRISPR–Cas9 engineering. Francisella novicida Cas9 (FnCas9) exhibits high intrinsic specificity but is constrained by stringent PAM requirements. Here, we...