Shigeyuki Kawano

Efficient targeted gene knock-in in Ulva using Cas9 RNPs and long single-stranded DNA donors

Abstract Background CRISPR-Cas9 ribonucleoprotein (RNP)-mediated genome editing has recently been established in the green seaweed Ulva . However, achieving precise and efficient targeted gene insertion remains challenging due to the low frequency of homology-directed repair (HDR...