Ruikang Tang

Native Mass Spectrometry-Based Proteoform Explicit Metrics System (PrEMS) Informs Structural Improvement of Artificial Photocyclase Enzymes

Abstract Directed evolution is a powerful strategy for enzyme engineering and has successfully enabled unique stereoselective biosynthesis of high-value organic molecules. Enantiomeric excess (ee) remains the gold standard to assess the enzyme performance, but only reflects the f...