Ralf F. W. Bartenschlager

Rapid and Efficient Affinity Isolation of Engineered Extracellular Vesicles Encapsulating Functional Proteins via Internal Twin‐Strep Tetraspanin Tagging

ABSTRACT Extracellular vesicles (EVs) are mediators of intercellular communication and promising protein‐delivery vehicles, but isolation of sufficiently pure, intact, engineered EVs remains challenging due to abundant cellular and serum‐derived contaminants. Here, structure‐guid...