Philipp T. Kaulich

Comprehensive Online Two-Dimensional nanoLCxCZE-MS for Deep Top-Down Proteomics

Abstract Understanding proteoforms, i.e., the various molecular forms in which proteins can exist, is important for deciphering biological processes and diseases. While capillary zone electrophoresis (CZE) proved advantageous for proteoform separation, limited sample loading capa...

Modulating proteoform charge states and signal intensities in LC-MS using a nanoflow sheath liquid interface

To investigate intact proteins in biological samples, hyphenation of mass spectrometers (MS) with separation techniques like liquid chromatography (LC) or capillary zone electrophoresis (CZE) is crucial. While reversed-phase nanoLC is typically coupled directly (“sheathless”) to...