Péter Nemes

Rapid Eco-AI CE–MS Enables Sub-7 Min Low-Input Proteome Profiling with Quantitative Validation

Abstract Low-input and single-cell proteomics by mass spectrometry (MS) have advanced rapidly, but balancing analytical speed and sensitivity remains challenging, especially for small, protein-scarce primary cells such as neutrophils. Here, we report Rapid Eco–AI, an optimized el...

AspirationPatch Proteomics Enables CE–ESI–MSProteotyping of Identified Single Neurons in Intact Brain Tissue

Abstract Direct proteome analysis of identified neurons in intact brain tissue remains limited by the difficulty of recovering intracellular material while preserving native tissue context and avoiding physical extraction of the intact soma. Here, we establish aspiration patch pr...