Pedro Ferro-Gallego

Proof-of-concept validation of p2GUS, a modular expression system for enhanced Ni-binding purification of functional recombinant proteins in Escherichia coli

Abstract Protein expression and purification remain critical steps in basic research, biotechnology, and industrial applications. Here, p2GUS, a modular Escherichia coli expression system generated through targeted modification of Precursor 1, a parental pBAD-derived vector, is d...