Na Wu

Mechanistic Decoupling of the cis - and trans -Cleavage Activities of Cas12a via Phosphorothioate-Induced Kinetic Trapping

Abstract CRISPR-Cas12a is widely utilized for genome engineering and nucleic acid diagnostics, being distinguished by its indiscriminate single-stranded DNA (ssDNA) trans-cleavage activity triggered by its specific cis-target recognition. However, the precise kinetic coordination...

State-dependent reconfiguration failure of the basal forebrain-cortical gradient in Parkinson’s disease with freezing of gait

Accumulating evidence implicates cholinergic dysfunction in freezing of gait (FOG) in Parkinson’s disease (PD). Given the basal forebrain (BF) provides the primary cholinergic input to the cortex, elucidating BF-cortical connectivity and its dynamic reconfiguration during motor c...