Michael L. Gross

TPOP: A Laser-Based, Peroxide-Free Approach for Microsecond Hydroxyl Radical Protein Footprinting in Water

Abstract We introduce here a novel protein footprinting approach termed “two-photon oxidation of proteins (TPOP)”. This method employs a nanosecond, high-power, 248 nm excimer laser to induce two-photon absorption in both water and aromatic amino acids within proteins. The two-ph...