Manting Jin

Transcriptome-informed selection and validation of reference genes for RT-qPCR normalization in Cuphea hookeriana across tissues and under low-temperature stress

Reverse transcription quantitative PCR (RT-qPCR) is widely used to quantify gene expression, but reliable normalization requires reference genes with stable expression in the species, tissues, and experimental conditions being examined. Cuphea hookeriana is an ornamental shrub wh...