Mǎng Shī

Library strategies differentially shape microbial, functional, and host signals in clinical metagenomic sequencing

Metagenomic next-generation sequencing (mNGS) is increasingly used in infectious disease diagnostics, yet how library preparation shapes the microbial, functional, and host signals recovered from clinical samples remains poorly defined. Here, we performed a within-sample parallel...

A total infectome framework for resolving complex disease etiology in aquaculture

Traditional pathogen isolation can establish disease causality but often fails in complex infections involving unknown or multiple microbial backgrounds, whereas metatranscriptomics comprehensively captures the total infectome without directly resolving the primary etiological ag...