Makoto Nishiyama

A repurposed pseudoenzyme enables redox- and acetylation-dependent control of CoA transferase

CoA transferases play essential roles in short-chain fatty acid and acyl-CoA metabolism, yet how their activities are regulated in response to metabolic signals remains unclear. Here, we show that a CoA transferase from Thermus thermophilus is controlled by a two-layer mechanism...

L-Glutamate production from nitrogen gas by co-culturing Corynebacterium glutamicum with Azotobacter vinelandii

Abstract Biological nitrogen fixation has attracted considerable attention as an alternative to Haber–Bosch-dependent fermentation processes and agricultural systems. Azotobacter vinelandii, a well-established model organism for aerobic nitrogen fixation, has been engineered to p...