Lifu Liu

An automated operon refactoring workflow for heterologous expression of non-ribosomal peptide biosynthetic gene clusters

Microbial genomes encode numerous biosynthetic gene clusters, yet most remain experimentally uncharacterized, particularly large non-ribosomal peptide synthetase (NRPS) clusters that resist conventional cloning. Existing refactoring approaches are labor-intensive, depend on de no...