Lidia Sas‐Paszt

Assessment of the survival rate of microorganisms in the soil and assessment of the soil microbial biodiversity with the use of the DGGE technique

Only a small proportion of microorganisms living in the soil environment start growing on nutrient media in the laboratory. For this reason, molecular techniques based on the analysis of DNA extracted directly from the soil are used to study the microbial diversity of soils. Thes...

Assessment of the survival rate of microorganisms in the soil and assessment of the soil microbial biodiversity -soil sampling

The application of beneficial microorganisms to the soil requires the control of their survival. The DGGE technique (Denaturating Gradient Gel Electrophoresis) is used for this purpose. The first step in the DGGE analysis is to collect soil samples.

Molecular identification of microorganisms: identification of microorganisms cultivated on microbiological media based on the analysis of gene sequences

This technique allows identification of genus/species of microorganisms based on the sequence of several, usually seven, selected loci, with the analysis involving the sequences of those genes that determine the basic functions of the cell. The data collected on the basis of sequ...

Molecular identification of microorganisms: identification of microorganisms cultivated on microbiological media based on the analysis of gene sequences

This technique allows identification of genus/species of microorganisms based on the sequence of several, usually seven, selected loci, with the analysis involving the sequences of those genes that determine the basic functions of the cell. The data collected on the basis of sequ...