Liangyu Zhu

A low-complexity, tissue-function-guided engineering workflow for AlphaFold 3-assisted affinity maturation of a CD21 diagnostic nanobody

High-affinity antibodies with reliable tissue-staining performance are needed for reproducible immunohistochemistry (IHC), yet affinity maturation workflows often optimize recombinant-antigen binding and treat staining in formalin-fixed, paraffin-embedded tissue as a downstream v...