Κωνσταντίνος Κωνσταντόπουλος

Protocol for optogenetic stimulation of cells under physical confinement

Optogenetic approaches enable spatiotemporal control of signaling proteins, yet their integration with microfluidic assays to study confined cell migration remains challenging. Herein, we present a protocol for the optogenetic activation of PI3K/Akt signaling in confined cells. W...