Kaili Nie

Stabilized 5-Hydroxymethylfurfural Oxidase and Kinetics-Guided Feeding Mitigate Substrate Inhibition in Resting-Cell 2,5-Furandicarboxylic Acid Production

Abstract HMFO enables O2-dependent conversion of 5-hydroxymethylfurfural (HMF) to 2,5-furandicarboxylic acid (FDCA) without soluble cofactor regeneration, but prolonged operation is limited by HMF substrate inhibition, pH drift, and enzyme deactivation. Here, these limitations we...