Jun Kunisawa

Carbohydrate-to-fat ratio is positively associated with comprehensive frailty across protein-intake levels in older adults

Abstract Purpose Diet influences frailty, and protein intake has been a prime focus of prevention. However, frailty remains prevalent, even among older adults with relatively high protein intake, suggesting the influence of remaining energy distribution between carbohydrates and...

Standardization of shotgun metagenomics parameters for pks-positive enterobacteria detection in human fecal samples: Receiver operating characteristic analysis using polymerase chain reaction as reference standard

Enterobacteria harboring the polyketide synthase ( pks ) island ( clbA – clbS ), which encodes colibactin ( pks⁺ enterobacteria), is a putative risk factor for colorectal cancer. Polymerase chain reaction (PCR) is the current standard for detection. Primer-independent shotgun met...