Jiacheng Wei

Untangling the Molecular Mechanism of SpCas9 HNH Domain Checkpoint Activation: A Gear-and-Wedge Fitting Model

Abstract The CRISPR-associated endonuclease Streptococcus pyogenes Cas9 (SpCas9) facilitates site-specific DNA cleavage, with structural transition from the checkpoint state to the catalytic conformation representing a critical activation step. This transition involves an approxi...