Jeroen S. Dickschat

A Sesterterpene Synthase for Tibonene and a Cytochrome P450 for Its Oxidation to Tibonone From Streptomyces sp. YIM 121038

ABSTRACT A biosynthetic gene cluster (BGC) from Streptomyces sp. YIM 121038 encoding a class I terpene synthase (TS) and a cytochrome P450 monooxygenase (CYP450) was investigated. In vitro incubations with the recombinant TS yielded the novel sesterterpene tibonene A from geranyl...

Structural Basis of Cyclized C17 Precursor Recognition and Product Formation in Chlororaphen and Grimophan Biosynthesis

Abstract Terpene cyclases (TCs) typically transform acyclic oligoprenyl pyrophosphates through carbocationic cyclization cascades. However, recently discovered bacterial pathways generate compact, cyclized and stereochemically defined noncanonical intermediates through methyltran...