Intawat Nookaew

Protocol for the enrichment of endosteal and periosteal mesenchymal cells from murine bone for single-cell transcriptome analysis

Bone comprises diverse cell populations essential for skeletal development, remodeling, and homeostasis. Current single-cell isolation methods often use bulk digestion that can obscure distinctions between periosteal and endosteal compartments. Here, we present an optimized murin...