Iara Plácido Guazzelli

Global analysis of thermal and chemical denaturation using CheMelt : Thermodynamic dissection of highly thermostable de novo designed proteins

ABSTRACT De novo protein design often produces thermostable proteins that denature above 100 °C, which complicates the analysis of their stability. Thermostable proteins can be unfolded by combined chemical and thermal denaturation followed by global analysis of multiple melting...