Hongtao Wen

CRISPR/Cas12a-Based Aptasensor for Cas9 Protein Detection in Spiked Food Matrices

Residual gene editors are a concern associated with the widespread application of CRISPR/Cas9 technology. Here, we developed a CRISPR/Cas12a-based fluorescent aptasensor for the qualitative and quantitative detection of Cas9 protein. Secondary structure prediction and molecular d...