Hanyu Zhu

Rational Engineering of Carboxylic Acid Reductase for Improved Catalytic Efficiency and Substrate Tolerance Enabling Green Vanillin Biosynthesis

Abstract Vanillin is a high-value natural aromatic compound widely used in food, flavor, and pharmaceutical fields. Carboxylic acid reductase (CAR)-catalyzed vanillic acid bioreduction offers a green vanillin biosynthesis route, yet low catalytic activity and severe substrate inh...