Fuming Liu

Field-deployable visual detection of Burkholderia gladioli and its toxigenic pathovar using a one-step RPA-CRISPR/Cas13a assay

ABSTRACT Burkholderia gladioli pathovar cocovenenans produces the potent mitochondrial toxin bongkrekic acid (BA), causing severe foodborne outbreaks. Conventional detection methods are constrained by prolonged turnaround times and the need for sophisticated instruments, limiting...