Farren J. Isaacs

The GndA Microprotein Promotes Cell Growth during Heat Shock in Escherichia coli

Abstract Hundreds of bacterial small open reading frames (sORFs) encoding microproteins of fewer than 50 amino acids have recently been identified. Biological functions have been ascribed to an increasing number of microproteins, some of which play roles in bacterial stress respo...

Engineered Orthogonal Translation Systems from Metagenomic Libraries Expand the Genetic Code

Abstract Genetic code expansion with noncanonical amino acids (ncAAs) opens new opportunities for the design and engineering of proteins by broadening their chemical repertoire. Unfortunately, ncAA incorporation into proteins is limited both by a small collection of orthogonal am...

Systematic prioritization of candidate genes in camptothecin biosynthesis using multi‐omics and deep learning

Abstract Camptothecin (CPT), a plant‐derived monoterpene indole alkaloid first identified in Camptotheca acuminata , is a drug precursor widely used for cancer chemotherapeutics. However, the full set of genes responsible for CPT biosynthesis remains unclear, hindering efforts to...