Erich Gnaiger

Protocol for isolation and cryopreservation of human peripheral blood mononuclear cells for high-resolution respirometry

High-resolution respirometry (HRR) of peripheral blood mononuclear cells (PBMCs) relies on controlled preanalytical procedures. Here, we present an optimized workflow for PBMC isolation from human whole blood, followed by short-term cryopreservation at −80°C. The protocol include...