Emmett Hanson

Photo-Activatable RNA Switches for Optical Control of Cell-Free Protein Synthesis

Abstract Programmable RNA switches enable target-dependent regulation of cell-free protein synthesis. Here, we report a photo-activatable RNA switch using photocleavable Lock DNA (pcl-Lock) to enable optical control of in vitro translation. Synthesized by solid-phase methods, pcl...

Programmable Genome Sensing with Visual, Fluorescence, and Glucometer Readings: Cell-Free In Vitro Translation Approach Using RNA Switch-CRISPR

Abstract Rapid and accessible detection of pathogens through diverse detection modalities remains highly desirable. Most current pathogen detection assays rely on a single detection modality. Here, we have used the same RNA switch-CRISPR platform for detection of multiple pathoge...