E. Lekka

Resolving the complex CES1 genomic locus with Cas9-directed targeted long-read sequencing

Abstract Carboxylesterase 1 (CES1) is a major hepatic enzyme involved in the metabolism of many ester-containing drugs and endogenous lipids. However, genomic analysis of the CES1 locus is challenging due to highly homologous regions that confound short-read sequencing. To overco...