Douglas A. Mitchell

An IscS Persulfide Donates the Sulfur for Ribosomal Protein uL16 Thioamidation

ABSTRACT Posttranslational sulfur incorporation into peptide backbones is increasingly reported. These efforts broaden our understanding of thioamides, which have distinct conformational and hydrogen‐bonding properties compared with amides. YcaO‐catalyzed thioamidation of the rib...

An IscS Persulfide Donates the Sulfur for Ribosomal Protein uL16 Thioamidation

ABSTRACT Posttranslational sulfur incorporation into peptide backbones is increasingly reported. These efforts broaden our understanding of thioamides, which have distinct conformational and hydrogen‐bonding properties compared with amides. YcaO‐catalyzed thioamidation of the rib...

Protein Thioether Crosslinks Installed by a SPASM-Containing Radical SAM Enzyme from Methanogenic Archaea

Abstract Cysteine is a vital amino acid with wide biological utility, serving functions in catalysis, ligand binding, and maintenance of protein structures. In a subset of natural products, termed ribosomally synthesized and post-translationally modified peptides (RiPPs), multipl...