Daniel B. Kearns

Efficient septum formation is essential for chromosome segregation in Bacillus subtilis when SMC function is impaired

ABSTRACT Structural maintenance of chromosomes (SMC) complexes play conserved roles in chromosome organization, segregation, and repair in all domains of life. In Bacillus subtilis , SMC is required for the segregation of newly replicated origins. To investigate whether other pro...

Constitutive, endogenous, fluorescent membrane reporters for dynamic cell cycle analysis in Bacillus subtilis

ABSTRACT Bacteria increase in biomass and divide, but determining precisely when cell division completes is technically challenging. To aid time-lapse imaging and cell-cycle tracking, we set out to identify a protein in Bacillus subtilis that, when fused with a fluorophore, would...