Daiki Asakawa

Cytochrome c is Highly Compact upon Transfer into the Gas Phase from Strong Acids as Revealed by High-Pressure ESI Ion Mobility-Mass Spectrometry

Abstract A substantial increase in the acidity of the protein solution (e.g., from pH 2 to pH 1) is known to induce the compaction of fully unfolded proteins, such as cytochrome c, into a non-native yet highly compact molten globule state, referred to as the A-state. In this stud...

Secondary Fragmentation of Benzylpyridinium Thermometer Ions Bearing a 4-Trifluoromethyl Group Extends the Internal Energy Calibration Range of Gas-Phase Ions

Abstract Secondary fragmentation of substituted benzylpyridinium “thermometer ions” has been observed previously, but they have not been used as quantitative probes for internal energy calibration. Here, we demonstrate that the secondary fragmentation of 4-trifluoromethyl-substit...