Author
Chloe Timmins-Young
Recent research
- BiologyOpen access
Protocol for eVLP-mediated CRISPR-Cas9 and prime editing in pancreatic cancer cell lines
Pancreatic ductal adenocarcinoma (PDAC) cell lines resist transfection, limiting gene-editing experiments. Here, we present a protocol for delivering CRISPR-Cas9 or prime editors into PDAC cell lines using retrovirus-derived engineered virus-like particles (eVLPs). We describe st...