Cheng Li

CEBPA-induced trophectoderm-like cells from pluripotent stem cells enable modeling of trophoblast lineage specification

Abstract Trophoblast lineages are essential for placental development and play indispensable roles in mammalian development. However, the regulatory mechanisms governing trophoblast lineage specification, particularly trophectoderm formation, remain elusive. Here, we show that tr...

Methyl-donor inhibitors relax 3D genome structures to promote totipotent reprogramming

The 3D genome safeguards cell identity while shaping developmental plasticity, yet its coordination with metabolism during cell fate transitions remains unclear. Here, we use a mouse totipotent stem cell induction system to dissect the role of methyl-donor metabolism in 3D genome...

Non-Destructive Chemoselective Labeling and Bioorthogonal Enrichment of Pseudouridine-Containing RNA via Facile N 1-Selective Functionalization

Abstract Pseudouridine (Ψ), often referred to as the “fifth nucleotide”, constitutes the most abundant post-transcriptional RNA modification and plays pivotal roles in regulating RNA structure and function. However, the precise mapping and functional investigation of Ψ remain cha...