Charles Yen

PCR Optimization of BOX-AIR PCR for Microbial Source Tracking of Escherichia coli in Waterways

Different PCR parameters and conditions were investigated to identify the optimal conditions for improved reproducibility and intensity of BOX A1R PCR products. Four isolates of Escherichia coli of either gull or duck origins were cultured, washed, and used as whole cell PCR temp...