Changwen Ye

Boosting Iturin A Production in Bacillus velezensis by Multimodule Engineering, Operon Restructuring, and Pickering Emulsion Fermentation

Abstract Iturin A is a valuable natural cyclic lipopeptide with broad application potential in multiple fields such as food preservation and agricultural production. However, its industrial production remains limited due to low fermentation yield. In this study, iturin A biosynth...

Mechanistic Decoupling of the cis - and trans -Cleavage Activities of Cas12a via Phosphorothioate-Induced Kinetic Trapping

Abstract CRISPR-Cas12a is widely utilized for genome engineering and nucleic acid diagnostics, being distinguished by its indiscriminate single-stranded DNA (ssDNA) trans-cleavage activity triggered by its specific cis-target recognition. However, the precise kinetic coordination...