Chandrika Sharma

Discovery and Modulation of an Unusual Terminal Mismatch Bypass Activity Involving 3′-Phosphorothioated Primers and Commercial Strand Displacement DNA Polymerases

Abstract Phosphorothioated (PST) oligonucleotides are extensively used in RNA therapeutics and biosensing applications. PST-modified linkages remain part of 13 approved oligonucleotide drug backbones (out of 23 total approved drugs). Despite the widespread utilization of PST link...