Bang‐Ce Ye

Rational Engineering of Carboxylic Acid Reductase for Improved Catalytic Efficiency and Substrate Tolerance Enabling Green Vanillin Biosynthesis

Abstract Vanillin is a high-value natural aromatic compound widely used in food, flavor, and pharmaceutical fields. Carboxylic acid reductase (CAR)-catalyzed vanillic acid bioreduction offers a green vanillin biosynthesis route, yet low catalytic activity and severe substrate inh...

Engineering a Transcription Activation Toolkit by Rewiring Prokaryotic Transcription Factors and Synthetic Yeast Promoters in Yarrowia lipolytica

Abstract Yarrowia lipolytica is a promising industrial host, yet its metabolic engineering potential remains limited by insufficient genetic tools. Here, we engineered a synthetic Transcription Activation Toolkit (TAT) based on LacI–VPRH, a chimeric protein fusing the prokaryotic...