Ayman M. Al‐Qaaneh

Comparison of manual counting with two rule-based image analysis programs, ImageJ and CellProfiler, for quantitative analysis of c-Fos immunohistochemical positivity in rat brainstem sections

Quantitative and semiquantitative assessment of biomarker expression using immunohistochemistry (IHC) can be performed using several analytical approaches. Manual counting remains widely used but is inherently subjective and susceptible to observer variability. Rule-based image-a...