Anto Rafael Sikirić

Beyond carbon dioxide: decoupling headspace and buffering reveals a bicarbonate requirement for anaerobic gut fungi growth

Abstract Background Anaerobic gut fungi ( Neocallimastigomycota ) are major lignocellulose degraders in herbivore gastrointestinal tracts, but reproducible axenic cultivation remains difficult because conventional serum-bottle systems intrinsically couple CO 2 headspace, bicarbon...