The new species, Mayorella budamensis, was identified using observations of living cells and comparisons of small-subunit ribosomal DNA, a genetic marker used to study relationships among organisms. The amoeba showed wide variation in cell size, shape, movement and floating forms, but its DNA distinguished it from the Mayorella species currently represented in sequence databases.

A second Mayorella isolate from the same stream formed another evolutionary lineage, although it was represented only by molecular data from a single cell and was not cultured. Broader analyses placed Mayorella into up to seven putative groups, most of which included unnamed or environmental lineages.